In previous years, microbiological inspections detected most often contaminants as follows increased total or aerobic mesophyllic bacteria, E. coli and faecal streptococcus, while chemical analyses detected organic contents, ammonia, nitrate, nitrite, iron, and other toxic matters (disinfection processes by products).

And the reason for this, it turned out, after two or three years' work, was that in the skin we have huge stores not of nitric oxide, because nitric oxide is a gas, and it's released and in a few seconds it's away, but it can be turned into these forms of nitric oxide nitrate, NO3 nitrite, NO2 nitrosothiols.